Sequence comparison of wild-type and cold-adapted B/Ann Arbor/1/66 influenza virus genes
Identifieur interne : 002205 ( Main/Exploration ); précédent : 002204; suivant : 002206Sequence comparison of wild-type and cold-adapted B/Ann Arbor/1/66 influenza virus genes
Auteurs : Dan C. Deborde [États-Unis] ; Armen M. Donabedian [États-Unis] ; M. Louise Herlocher [États-Unis] ; Clayton W. Naeve [États-Unis] ; Hunein F. Maassab [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1988.
English descriptors
- Teeft :
- Acid change, Acid changes, Adenosine residues, Amino, Amino acid, Amino acid change, Amino acid changes, Amino acid substitutions, Amino acids, Attenuated virulence, Base substitutions, Bethesda research laboratories, Better chance, Briedis, Chemical sequencing reactions, Coding region, Codon, Cold adaptation process, Consensus sequence, Consensus sequences, Constant voltage, Convergent changes, Crna, Deborde, Donabedian, Donor virus, Ethidium bromide, Functional constraint, Gene, Gene pairs, Gene sequence, Gene sequences, Genetic studies, Genome, High degree, High dilution, Identical conventions, Individual gene, Individual viruses, Influenza, Influenza type, Influenza virus, Influenza viruses, Life sciences, Maassab, Mutation, Mutation rate, Nonlethal mutations, Nonsurface, Nonsurface genes, Nonunique, Nonunique changes, Nonunique nucleotide changes, Nucleic acids, Nucleotide, Nucleotide changes, Nucleotide substitutions, Open reading frame, Other genes, Phenotype, Plaquing, Plaquing step, Polypeptide sequences, Primer, Primer location, Protein protein, Reading frame, Reassortant, Reassortant vaccines, Reassortants, Sequence changes, Sequence data, Sequence differences, Sequencing, Sodium acetate, Sodium borohydride, Specific phenotypes, Substitution, Terminal deoxynucleotidyl transferase, Tissue culture, Total number, Unique nucleotide changes, Uridine nucleotides, Vaccine, Vaccine reassortants, Vacuum concentrator, Viral, Viral genes, Virology, Virus, Virus consensus sequence, Virus genome, Virus pool, Virus sequence, Virus vaccine, Vrna, Vrna segments.
Abstract
Abstract: Consensus sequences for both wt and ca B/Ann Arbor/1 /66 viral PB2, PB1, PA, NP, M, and NS genes were directly determined from vRNA using a combination of chemical and chain-termination sequencing methods. There were 105 sites of difference between the wt and ca sets of these six RNA genes. The differences resulted in 26 amino acid substitutions distributed over the six proteins. The sequence changes were compared to the sequences of other known influenza type B wt viruses to pinpoint those changes that were unique to the ca B/Ann Arbor/1/66 virus. Of the 26 amino acid differences, only 11 were unique to the cold-adapted virus. These unique sites were distributed among five of the six genes. The NS protein had no amino acid substitutions. The sequence changes are discussed in terms of their probable mode of origin and selection, and in terms of their importance to the cold-adapted, temperature-sensitive, and attenuation phenotypes of ca B/AA/1 /66 virus. The sequence and organization of the PB2 gene and predicted protein are also given. The PB2 gene was 2396 nucleotides long, and it encoded a predicted protein of 770 amino acids with a molecular weight of 88,035 Da for the wt virus and 88,072 Da for the ca virus. Both proteins were predominantly hydrophilic, and each had an overall charge of +24.5 at pH 7.0.
Url:
DOI: 10.1016/0042-6822(88)90284-X
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001577
- to stream Istex, to step Curation: 001577
- to stream Istex, to step Checkpoint: 000F18
- to stream Main, to step Merge: 002313
- to stream Main, to step Curation: 002205
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Sequence comparison of wild-type and cold-adapted B/Ann Arbor/1/66 influenza virus genes</title><author><name sortKey="Deborde, Dan C" sort="Deborde, Dan C" uniqKey="Deborde D" first="Dan C." last="Deborde">Dan C. Deborde</name></author><author><name sortKey="Donabedian, Armen M" sort="Donabedian, Armen M" uniqKey="Donabedian A" first="Armen M." last="Donabedian">Armen M. Donabedian</name></author><author><name sortKey="Herlocher, M Louise" sort="Herlocher, M Louise" uniqKey="Herlocher M" first="M. Louise" last="Herlocher">M. Louise Herlocher</name></author><author><name sortKey="Naeve, Clayton W" sort="Naeve, Clayton W" uniqKey="Naeve C" first="Clayton W." last="Naeve">Clayton W. Naeve</name></author><author><name sortKey="Maassab, Hunein F" sort="Maassab, Hunein F" uniqKey="Maassab H" first="Hunein F." last="Maassab">Hunein F. Maassab</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:3928AD04942CFCF4F75529E619B85C039A46E8F4</idno><date when="1988" year="1988">1988</date><idno type="doi">10.1016/0042-6822(88)90284-X</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-X60RM77C-H/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001577</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001577</idno><idno type="wicri:Area/Istex/Curation">001577</idno><idno type="wicri:Area/Istex/Checkpoint">000F18</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F18</idno><idno type="wicri:doubleKey">0042-6822:1988:Deborde D:sequence:comparison:of</idno><idno type="wicri:Area/Main/Merge">002313</idno><idno type="wicri:Area/Main/Curation">002205</idno><idno type="wicri:Area/Main/Exploration">002205</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Sequence comparison of wild-type and cold-adapted B/Ann Arbor/1/66 influenza virus genes</title><author><name sortKey="Deborde, Dan C" sort="Deborde, Dan C" uniqKey="Deborde D" first="Dan C." last="Deborde">Dan C. Deborde</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, School of Public Health, University of Michigan, 109 Observatory St., Ann Arbor, Michigan, 48109</wicri:regionArea><wicri:noRegion>48109</wicri:noRegion></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Montana</region></placeName><wicri:cityArea>1 Present address: Department of Microbiology, University of Montana, Missoula</wicri:cityArea></affiliation></author><author><name sortKey="Donabedian, Armen M" sort="Donabedian, Armen M" uniqKey="Donabedian A" first="Armen M." last="Donabedian">Armen M. Donabedian</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, School of Public Health, University of Michigan, 109 Observatory St., Ann Arbor, Michigan, 48109</wicri:regionArea><wicri:noRegion>48109</wicri:noRegion></affiliation></author><author><name sortKey="Herlocher, M Louise" sort="Herlocher, M Louise" uniqKey="Herlocher M" first="M. Louise" last="Herlocher">M. Louise Herlocher</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, School of Public Health, University of Michigan, 109 Observatory St., Ann Arbor, Michigan, 48109</wicri:regionArea><wicri:noRegion>48109</wicri:noRegion></affiliation></author><author><name sortKey="Naeve, Clayton W" sort="Naeve, Clayton W" uniqKey="Naeve C" first="Clayton W." last="Naeve">Clayton W. Naeve</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee, 38101</wicri:regionArea><wicri:noRegion>38101</wicri:noRegion></affiliation></author><author><name sortKey="Maassab, Hunein F" sort="Maassab, Hunein F" uniqKey="Maassab H" first="Hunein F." last="Maassab">Hunein F. Maassab</name><affiliation></affiliation><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, School of Public Health, University of Michigan, 109 Observatory St., Ann Arbor, Michigan, 48109</wicri:regionArea><wicri:noRegion>48109</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1988">1988</date><biblScope unit="volume">163</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="429">429</biblScope><biblScope unit="page" to="443">443</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acid change</term><term>Acid changes</term><term>Adenosine residues</term><term>Amino</term><term>Amino acid</term><term>Amino acid change</term><term>Amino acid changes</term><term>Amino acid substitutions</term><term>Amino acids</term><term>Attenuated virulence</term><term>Base substitutions</term><term>Bethesda research laboratories</term><term>Better chance</term><term>Briedis</term><term>Chemical sequencing reactions</term><term>Coding region</term><term>Codon</term><term>Cold adaptation process</term><term>Consensus sequence</term><term>Consensus sequences</term><term>Constant voltage</term><term>Convergent changes</term><term>Crna</term><term>Deborde</term><term>Donabedian</term><term>Donor virus</term><term>Ethidium bromide</term><term>Functional constraint</term><term>Gene</term><term>Gene pairs</term><term>Gene sequence</term><term>Gene sequences</term><term>Genetic studies</term><term>Genome</term><term>High degree</term><term>High dilution</term><term>Identical conventions</term><term>Individual gene</term><term>Individual viruses</term><term>Influenza</term><term>Influenza type</term><term>Influenza virus</term><term>Influenza viruses</term><term>Life sciences</term><term>Maassab</term><term>Mutation</term><term>Mutation rate</term><term>Nonlethal mutations</term><term>Nonsurface</term><term>Nonsurface genes</term><term>Nonunique</term><term>Nonunique changes</term><term>Nonunique nucleotide changes</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nucleotide changes</term><term>Nucleotide substitutions</term><term>Open reading frame</term><term>Other genes</term><term>Phenotype</term><term>Plaquing</term><term>Plaquing step</term><term>Polypeptide sequences</term><term>Primer</term><term>Primer location</term><term>Protein protein</term><term>Reading frame</term><term>Reassortant</term><term>Reassortant vaccines</term><term>Reassortants</term><term>Sequence changes</term><term>Sequence data</term><term>Sequence differences</term><term>Sequencing</term><term>Sodium acetate</term><term>Sodium borohydride</term><term>Specific phenotypes</term><term>Substitution</term><term>Terminal deoxynucleotidyl transferase</term><term>Tissue culture</term><term>Total number</term><term>Unique nucleotide changes</term><term>Uridine nucleotides</term><term>Vaccine</term><term>Vaccine reassortants</term><term>Vacuum concentrator</term><term>Viral</term><term>Viral genes</term><term>Virology</term><term>Virus</term><term>Virus consensus sequence</term><term>Virus genome</term><term>Virus pool</term><term>Virus sequence</term><term>Virus vaccine</term><term>Vrna</term><term>Vrna segments</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Consensus sequences for both wt and ca B/Ann Arbor/1 /66 viral PB2, PB1, PA, NP, M, and NS genes were directly determined from vRNA using a combination of chemical and chain-termination sequencing methods. There were 105 sites of difference between the wt and ca sets of these six RNA genes. The differences resulted in 26 amino acid substitutions distributed over the six proteins. The sequence changes were compared to the sequences of other known influenza type B wt viruses to pinpoint those changes that were unique to the ca B/Ann Arbor/1/66 virus. Of the 26 amino acid differences, only 11 were unique to the cold-adapted virus. These unique sites were distributed among five of the six genes. The NS protein had no amino acid substitutions. The sequence changes are discussed in terms of their probable mode of origin and selection, and in terms of their importance to the cold-adapted, temperature-sensitive, and attenuation phenotypes of ca B/AA/1 /66 virus. The sequence and organization of the PB2 gene and predicted protein are also given. The PB2 gene was 2396 nucleotides long, and it encoded a predicted protein of 770 amino acids with a molecular weight of 88,035 Da for the wt virus and 88,072 Da for the ca virus. Both proteins were predominantly hydrophilic, and each had an overall charge of +24.5 at pH 7.0.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Montana</li></region></list><tree><country name="États-Unis"><noRegion><name sortKey="Deborde, Dan C" sort="Deborde, Dan C" uniqKey="Deborde D" first="Dan C." last="Deborde">Dan C. Deborde</name></noRegion><name sortKey="Deborde, Dan C" sort="Deborde, Dan C" uniqKey="Deborde D" first="Dan C." last="Deborde">Dan C. Deborde</name><name sortKey="Donabedian, Armen M" sort="Donabedian, Armen M" uniqKey="Donabedian A" first="Armen M." last="Donabedian">Armen M. Donabedian</name><name sortKey="Herlocher, M Louise" sort="Herlocher, M Louise" uniqKey="Herlocher M" first="M. Louise" last="Herlocher">M. Louise Herlocher</name><name sortKey="Maassab, Hunein F" sort="Maassab, Hunein F" uniqKey="Maassab H" first="Hunein F." last="Maassab">Hunein F. Maassab</name><name sortKey="Naeve, Clayton W" sort="Naeve, Clayton W" uniqKey="Naeve C" first="Clayton W." last="Naeve">Clayton W. Naeve</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002205 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002205 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:3928AD04942CFCF4F75529E619B85C039A46E8F4
|texte= Sequence comparison of wild-type and cold-adapted B/Ann Arbor/1/66 influenza virus genes
}}
| This area was generated with Dilib version V0.6.33. |  |